Method for determining the presence of reagin-immunoglobulins(reagin-ig)directed against certain allergens,in aqueous samples

ABSTRACT

AN IN VITRO METHOD FOR ANALYZING A BODY FLUID FOR REAGIN-IMMUNOGLOBULINS THEREIN THAT ARE REACTIVE WITH ALLERGENS WHICH COMPRISES CONTACTING A SAMPLE OF BODY FLUID WITH A WATER-INSOLUBLE POLYMER TO WHICH IS ATTACHED AT LEAST ONE TEST ALLERGEN FOR A LONG ENOUGH TIME TO ALLOW REACTION THEREBETWEEN AND THEN CONTACTING WITH EITHER LABELLED ANTIBODIES THAT ARE REACTIVE WITH THE REAGIN IMMUNOGLOBULINS OR WITH ANTIBODIES AGAINST REAGIN-IG AND REAGIN-IG LABELLED WITH A RADIATION EMITTING ATOM OR GROUP, SEPARATING INTO A LIQUID PHASE AND A SOLID PHASE, AND MEASURING THE RADIATION EMITTED FROM AT LEAST ONE OF THE TWO SEPARATED PHASES.



